BIOCHEMICAL AND GEOGRAPHICAL STUDIES ON POTATO TUBER MOTH, PHTHORIMAEA OPERCULELLA (ZELLER) IN SOME GOVERNORATES OF ARAB REPUBLIC OF EGYPT

Simple proteins, lipids and carbohydrates were examined by acrylamide –gel electrophoresis in the whole body( larva or prepupa ,pupa and adult) of the potato tuber moth Phthorimaea operculella in three governorates in the Delta of Egypt . Polymorphic in the whole body of potato tuber moth, variations in simple proteins ,lipoproteins ,lipoglycoproteins according to their mobility on polyacrylamide gel electrophoresis and staining with Coomassie brilliant blue ® ,Sudan black B and Periodic acid Schiff’s were detected. Twenty bands were further characterized simple proteins, lipoproteins, glycoproteins, lipoglycoproteins. The different protein concentrations was stage and locations dependent it reached a peak just before the last molt .Sodium dodecyl - sulphate – polyacrylamide gel electrophoresis (SDS-PAGE) resolved the protein into single polypeptide with molecular weight in the three governorates tested in Delta of Egypt ranged between209kDaltons to 19.37 k Daltons